Neo-CODA | PMID 16428827

Daidzein activates choline acetyltransferase from MC-IXC cells and improves drug-induced amnesia.

PMID 16428827 · Relations processed 2025-10-13T15:41:19.260000

Title

Daidzein activates choline acetyltransferase from MC-IXC cells and improves drug-induced amnesia.

Abstract

The choline acetyltransferase (ChAT) activator, which enhances cholinergic transmission via an augmentation of the enzymatic production of acetylcholine (ACh), is an important factor in the treatment of Alzheimer's disease (AD). Methanolic extracts from Pueraria thunbergiana exhibited an activation effect (46%) on ChAT in vitro. Via the sequential isolation of Pueraria thunbergiana, the active component was ultimately identified as daidzein (4',7-dihydroxy-isoflavone). In order to investigate the effects of daidzein from Pueraria thunbergiana on scopolamine-induced impairments of learning and memory, we conducted a series of in vivo tests. Administration of daidzein (4.5 mg/kg body weight) to mice was shown significantly to reverse scopolamine-induced amnesia, according to the results of a Y-maze test. Injections of scopolamine into mice resulted in impaired performance on Y-maze tests (a 37% decreases in alternation behavior). By way of contrast, mice treated with daidzein prior to the scopolamine injections were noticeably protected from this performance impairment (an approximately 12%-21% decrease in alternation behavior). These results indicate that daidzein might play a role in acetylcholine biosynthesis as a ChAT activator, and that it also ameliorates scopolamine-induced amnesia.

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Summary

Entities: 7 Relations: 14

NEGATIVE_CORRELATION: 8 POSITIVE_CORRELATION: 6

chemical (3) disease (2) gene (2)

Entity Breakdown

Type	Count
chemical	3
disease	2
gene	2

Normalized Entities

Entity	Type	Source	ID	Mentions	Surface forms
Isoflavones	chemical	mesh	`D007529`	7	4',7-dihydroxy-isoflavone, Daidzein, daidzein
Acetylcholine	chemical	mesh	`D000109`	3	ACh, acetylcholine
Scopolamine	chemical	mesh	`D012601`	5	scopolamine
Alzheimer Disease	disease	mesh	`D000544`	2	AD, Alzheimer's disease
Amnesia, Anterograde	disease	mesh	`D020324`	2	scopolamine-induced amnesia
Choline acetyltransferase	gene	ncbi_gene	`42249`	3	ChAT
choline O-acetyltransferase	gene	ncbi_gene	`1103`	2	choline acetyltransferase

Relations

Subject	Relation	Object
chemical Isoflavones mesh: `D007529` Surface: 4',7-dihydroxy-isoflavone, Daidzein, daidzein Mentions: 7	POSITIVE_CORRELATION	gene Choline acetyltransferase ncbi_gene: `42249` Surface: ChAT Mentions: 3 Context The passage states that daidzein activates choline acetyltransferase (ChAT), indicating a chemical-to-gene positive correlation.
chemical Isoflavones mesh: `D007529` Surface: 4',7-dihydroxy-isoflavone, Daidzein, daidzein Mentions: 7	NEGATIVE_CORRELATION	disease Amnesia, Anterograde mesh: `D020324` Surface: scopolamine-induced amnesia Mentions: 2 Context The passage states that administration of daidzein reversed scopolamine-induced amnesia and protected mice from performance impairment, indicating daidzein treats the disease.
chemical Isoflavones mesh: `D007529` Surface: 4',7-dihydroxy-isoflavone, Daidzein, daidzein Mentions: 7	NEGATIVE_CORRELATION	disease Alzheimer Disease mesh: `D000544` Surface: AD, Alzheimer's disease Mentions: 2 Context The passage states that daidzein activates choline acetyltransferase, which is described as an important factor in the treatment of Alzheimer's disease, indicating a treatment (negative correlation) relationship between the chemical and the disease.
chemical Scopolamine mesh: `D012601` Surface: scopolamine Mentions: 5	POSITIVE_CORRELATION	disease Amnesia, Anterograde mesh: `D020324` Surface: scopolamine-induced amnesia Mentions: 2 Context The passage states that injections of scopolamine into mice resulted in impaired performance and describes scopolamine-induced amnesia, indicating the chemical scopolamine induces the disease amnesia.
chemical Isoflavones mesh: `D007529` Surface: 4',7-dihydroxy-isoflavone, Daidzein, daidzein Mentions: 7	POSITIVE_CORRELATION	gene choline O-acetyltransferase ncbi_gene: `1103` Surface: choline acetyltransferase Mentions: 2 Context The passage states that daidzein activates choline acetyltransferase, indicating a chemical (daidzein) positively influences the gene product (choline acetyltransferase).
disease Amnesia, Anterograde mesh: `D020324` Surface: scopolamine-induced amnesia Mentions: 2	NEGATIVE_CORRELATION	gene Choline acetyltransferase ncbi_gene: `42249` Surface: ChAT Mentions: 3 Context The passage states that activation of ChAT (gene) ameliorates scopolamine-induced amnesia (disease), indicating that ChAT activity reduces the disease.
chemical Acetylcholine mesh: `D000109` Surface: ACh, acetylcholine Mentions: 3	NEGATIVE_CORRELATION	disease Alzheimer Disease mesh: `D000544` Surface: AD, Alzheimer's disease Mentions: 2 Context The passage states that acetylcholine (ACh) is an important factor in the treatment of Alzheimer's disease, indicating the chemical is used to treat the disease.
disease Amnesia, Anterograde mesh: `D020324` Surface: scopolamine-induced amnesia Mentions: 2	NEGATIVE_CORRELATION	gene choline O-acetyltransferase ncbi_gene: `1103` Surface: choline acetyltransferase Mentions: 2 Context The passage states that activation of choline acetyltransferase (gene) ameliorates scopolamine-induced amnesia (disease), indicating the gene is used to treat/prevent the disease.
chemical Isoflavones mesh: `D007529` Surface: 4',7-dihydroxy-isoflavone, Daidzein, daidzein Mentions: 7	NEGATIVE_CORRELATION	chemical Scopolamine mesh: `D012601` Surface: scopolamine Mentions: 5 Context The passage states that daidzein reverses scopolamine‑induced amnesia and protects mice from scopolamine‑induced performance impairment, indicating daidzein decreases the effect of scopolamine.
disease Alzheimer Disease mesh: `D000544` Surface: AD, Alzheimer's disease Mentions: 2	NEGATIVE_CORRELATION	gene Choline acetyltransferase ncbi_gene: `42249` Surface: ChAT Mentions: 3 Context The passage states that choline acetyltransferase (ChAT) is an important factor in the treatment of Alzheimer's disease, indicating the gene is used to treat the disease.
chemical Acetylcholine mesh: `D000109` Surface: ACh, acetylcholine Mentions: 3	POSITIVE_CORRELATION	gene Choline acetyltransferase ncbi_gene: `42249` Surface: ChAT Mentions: 3 Context The passage states that ChAT (gene) activation augments enzymatic production of acetylcholine, indicating a positive correlation between the chemical acetylcholine and the gene ChAT.
chemical Isoflavones mesh: `D007529` Surface: 4',7-dihydroxy-isoflavone, Daidzein, daidzein Mentions: 7	POSITIVE_CORRELATION	chemical Acetylcholine mesh: `D000109` Surface: ACh, acetylcholine Mentions: 3 Context The passage states that daidzein activates choline acetyltransferase, leading to an augmentation of acetylcholine production, indicating daidzein increases acetylcholine levels.
disease Alzheimer Disease mesh: `D000544` Surface: AD, Alzheimer's disease Mentions: 2	NEGATIVE_CORRELATION	gene choline O-acetyltransferase ncbi_gene: `1103` Surface: choline acetyltransferase Mentions: 2 Context The passage states that choline acetyltransferase (ChAT) is an important factor in the treatment of Alzheimer's disease, indicating the gene is used to treat/prevent the disease.
chemical Acetylcholine mesh: `D000109` Surface: ACh, acetylcholine Mentions: 3	POSITIVE_CORRELATION	gene choline O-acetyltransferase ncbi_gene: `1103` Surface: choline acetyltransferase Mentions: 2 Context The passage states that choline acetyltransferase enhances enzymatic production of acetylcholine, indicating a positive association between the chemical acetylcholine and the gene choline acetyltransferase.